Tomato hybrid drth1034 and parents thereof

ABSTRACT

The invention provides seeds and plants of tomato hybrid DRTH1034, tomato line FIR-XM20-4542, and tomato line FIR-XM20-4543. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTH1034, tomato line FIR-XM20-4542, and tomato line FIR-XM20-4543 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another plant, such as a tomato plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTH1034, tomato line FIR-XM20-4542, and tomato line FIR-XM20-4543 comprising introduced beneficial or desirable traits.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the priority of U.S. Provisional Appl. Ser. No.63/300,855, filed Jan. 19, 2022, the entire disclosure of which isincorporated herein by reference.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of tomato hybrid DRTH1034, tomato lineFIR-XM20-4542, and tomato line FIR-XM20-4543.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety. Such desirable traits may include any trait deemedbeneficial or desirable by a grower or consumer, including greateryield, resistance to insects or disease, tolerance to environmentalstress, and nutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all genetic loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for manygenetic loci. Conversely, a cross of two plants each heterozygous at anumber of loci produces a population of hybrid plants that differgenetically and are not uniform. The resulting non-uniformity makesperformance unpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a tomato plant of hybridDRTH1034, line FIR-XM20-4542 or line FIR-XM20-4543. Also provided aretomato plants having all the physiological and morphologicalcharacteristics of such a plant. Parts of these tomato plants are alsoprovided, for example, including pollen, an ovule, an embryo, a seed, ascion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of tomato hybrid DRTH1034,tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543 comprising anadded heritable trait is provided. The heritable trait may comprise agenetic locus that is, for example, a dominant or recessive allele. Inone embodiment of the invention, a plant of tomato hybrid DRTH1034,tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543 is defined ascomprising a single locus conversion. In specific embodiments of theinvention, an added genetic locus confers one or more traits such as,for example, herbicide tolerance, insect resistance, disease resistance,and modified carbohydrate metabolism. In further embodiments, the traitmay be conferred by a naturally occurring gene introduced into thegenome of a line by backcrossing, a natural or induced mutation, or atransgene introduced through genetic transformation techniques into theplant or a progenitor of any previous generation thereof. Whenintroduced through transformation, a genetic locus may comprise one ormore genes integrated at a single chromosomal location.

In some embodiments, a single locus conversion includes one or moresite-specific changes to the plant genome, such as, without limitation,one or more nucleotide modifications, deletions, or insertions. A singlelocus may comprise one or more genes or nucleotides integrated ormutated at a single chromosomal location. In one embodiment, a singlelocus conversion may be introduced by a genetic engineering technique,methods of which include, for example, genome editing with engineerednucleases (GEEN). Engineered nucleases include, but are not limited to,Cas endonucleases; zinc finger nucleases (ZFNs); transcriptionactivator-like effector nucleases (TALENs); engineered meganucleases,also known as homing endonucleases; and other endonucleases for DNA orRNA-guided genome editing that are well-known to the skilled artisan.

The invention also concerns the seed of tomato hybrid DRTH1034, tomatoline FIR-XM20-4542, or tomato line FIR-XM20-4543. The seed of theinvention may be provided as an essentially homogeneous population ofseed of tomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomatoline FIR-XM20-4543. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, seedof tomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543 may be defined as forming at least about 97% of the totalseed, including at least about 98%, 99%, or more of the seed. The seedpopulation may be separately grown to provide an essentially homogeneouspopulation of tomato plants designated DRTH1034, FIR-XM20-4542, orFIR-XM20-4543.

In yet another aspect of the invention, a tissue culture of regenerablecells of a tomato plant of hybrid DRTH1034, tomato line FIR-XM20-4542,or tomato line FIR-XM20-4543 is provided. The tissue culture willpreferably be capable of regenerating tomato plants capable ofexpressing all of the physiological and morphological characteristics ofthe starting plant and of regenerating plants having substantially thesame genotype as the starting plant. Examples of some of thephysiological and morphological characteristics of tomato hybridDRTH1034, tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543include those traits set forth in the tables herein. The regenerablecells in such tissue cultures may be derived, for example, from embryos,meristems, cotyledons, pollen, leaves, anthers, roots, root tips,pistils, flowers, seed, and stalks. Still further, the present inventionprovides tomato plants regenerated from a tissue culture of theinvention, the plants having all the physiological and morphologicalcharacteristics of tomato hybrid DRTH1034, tomato line FIR-XM20-4542, ortomato line FIR-XM20-4543.

In still yet another aspect of the invention, processes are provided forproducing tomato seeds, plants, and fruit, which processes generallycomprise crossing a first parent tomato plant with a second parenttomato plant, wherein at least one of the first or second parent plantsis a plant of tomato line FIR-XM20-4542 or tomato line FIR-XM20-4543.These processes may be further exemplified as processes for preparinghybrid tomato seed or plants, wherein a first tomato plant is crossedwith a second tomato plant of a different, distinct genotype to providea hybrid that has, as one of its parents, a plant of tomato lineFIR-XM20-4542 or tomato line FIR-XM20-4543. In these processes, crossingwill result in the production of seed. The seed production occursregardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent tomato plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent tomato plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent tomato plants. Yet another step comprisesharvesting the seeds from at least one of the parent tomato plants. Theharvested seed can be grown to produce a tomato plant or hybrid tomatoplant.

The present invention also provides the tomato seeds and plants producedby a process that comprises crossing a first parent tomato plant with asecond parent tomato plant, wherein at least one of the first or secondparent tomato plants is a plant of tomato hybrid DRTH1034, tomato lineFIR-XM20-4542, or tomato line FIR-XM20-4543. In one embodiment of theinvention, tomato seed and plants produced by the process are firstgeneration (F₁) hybrid tomato seed and plants produced by crossing aplant in accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridtomato plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid tomato plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from tomato hybrid DRTH1034, tomato lineFIR-XM20-4542, or tomato line FIR-XM20-4543, the method comprising thesteps of: (a) preparing a progeny plant derived from tomato hybridDRTH1034, tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543,wherein said preparing comprises crossing a plant of tomato hybridDRTH1034, tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543 with asecond plant; and (b) crossing the progeny plant with itself or a secondplant to produce a seed of a progeny plant of a subsequent generation.In further embodiments, the method may additionally comprise: (c)growing a progeny plant of a subsequent generation from said seed of aprogeny plant of a subsequent generation and crossing the progeny plantof a subsequent generation with itself or a second plant; and repeatingthe steps for an additional 3-10 generations to produce a plant derivedfrom tomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543. The plant derived from tomato hybrid DRTH1034, tomatoline FIR-XM20-4542, or tomato line FIR-XM20-4543 may be an inbred line,and the aforementioned repeated crossing steps may be defined ascomprising sufficient inbreeding to produce the inbred line. In themethod, it may be desirable to select particular plants resulting fromstep (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom tomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543 is obtained which possesses some of the desirable traitsof the line/hybrid as well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of tomatohybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543, wherein the plant has been cultivated to maturity, and(b) collecting at least one tomato from the plant.

In still yet another aspect of the invention, the genetic complement oftomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543 is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a tomato plant,or a cell or tissue of that plant. A genetic complement thus representsthe genetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make-up of a hybrid cell, tissue orplant. The invention thus provides tomato plant cells that have agenetic complement in accordance with the tomato plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that tomato hybrid DRTH1034, tomato line FIR-XM20-4542,or tomato line FIR-XM20-4543 could be identified by any of the manywell-known techniques such as, for example, Simple Sequence LengthPolymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 18:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by tomato plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a tomato plant of the invention with a haploid geneticcomplement of a second tomato plant, preferably, another, distincttomato plant. In another aspect, the present invention provides a tomatoplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have,” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes,” and“including,” are also open-ended. For example, any method that“comprises,” “has,” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has,” or “includes” oneor more traits is not limited to possessing only those one or moretraits and covers other unlisted traits.

Other objects, features, and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds, and derivatives of tomato hybrid DRTH1034, tomato lineFIR-XM20-4542, and tomato line FIR-XM20-4543.

Tomato hybrid DRTH1034, also known as PRODICE and 20-XM-FIR-1034, is anindeterminate tomato variety that develops a plant that produces large,red fruit. Tomato hybrid DRTH1034 comprises resistance to Fusariumoxysporum f sp. lycopersici, Fulvia fulvum, Stemphylium spp., Oidiumneolycopersicum, Tomato Mosaic Tobamovirus, Tomato Spotted Wilt Virus,and Tomato Torrado Virus.

Tomato line FIR-XM20-4542 is an indeterminate tomato variety thatproduces large, red fruit. Tomato line FIR-XM20-4542 comprisesresistance to Fusarium oxysporum f sp. lycopersici, Fulvia fulvum,Stemphylium spp., Tomato Mosaic Tobamovirus, Tomato Spotted Wilt Virus,and Tomato Torrado Virus.

Tomato line FIR-XM20-4543 is an indeterminate tomato variety thatproduces medium, red fruit. FIR-XM20-4543 comprises resistance toFusarium oxysporum f sp. lycopersici, Fulvia fulvum, Stemphylium spp.,Oidium neolycopersicum, Tomato Mosaic Tobamovirus, and Tomato TorradoVirus.

A. Origin and Breeding History of Tomato Hybrid DRTH1034

The parents of tomato hybrid DRTH1034 are tomato line FIR-XM20-4542 andtomato line FIR-XM20-4543. The parent lines are uniform and stable, asis a hybrid produced therefrom. A small percentage of variants can occurwithin commercially acceptable limits for almost any characteristicduring the course of repeated multiplication. However no variants areexpected.

B. Physiological and Morphological Characteristics of Tomato HybridDRTH1034, Tomato Line FIR-XM20-4542, and Tomato Line FIR-XM20-4543

In accordance with one aspect of the present invention, there areprovided plants having the physiological and morphologicalcharacteristics of tomato hybrid DRTH1034 and the parent lines thereof.Descriptions of the physiological and morphological characteristics ofsuch plants are presented in the tables that follow.

TABLE 1 Physiological and Morphological Characteristics of Tomato HybridDRTH1034 CHARACTERISTIC DRTH1034 Marinice Seedling anthocyanin inhypocotyl present present of 2-15 cm seedling habit of 3-4 week oldnormal normal seedling Mature Plant height (cm) 194.9 181.3 height longlong growth type indeterminate indeterminate form normal normal canopysize (compared medium medium to others of similar type) habit erecterect Stem anthocyanin coloration weak medium of upper third length ofinternode short short branching sparse sparse branching at cotyledon orpresent present first leafy node number of nodes between 7 to 10 7 to 10first inflorescence number of nodes between  3.0  3.0 early (first tosecond, second to third) inflorescences number of nodes between  3.0 3.0 later developing inflorescences pubescence on younger sparselyhairy moderately hairy stems (scattered long hairs) Leaf type (matureleaf beneath tomato tomato the third inflorescence) type of bladebipinnate bipinnate margins of major leaflets shallowly toothed deeplytoothed or (mature leaf beneath the or scalloped cut, sps. toward thirdinflorescence) base marginal rolling or absent absent wiltiness (matureleaf beneath the third inflorescence) surface of major leaflets rugoserugose (mature leaf beneath the (bumpy or (bumpy or third inflorescence)veiny) veiny) pubescence (mature leaf normal normal beneath the thirdinflorescence) attitude horizontal horizontal length medium medium widthbroad broad size of leaflets large large intensity of green color mediummedium to dark glossiness medium medium blistering weak weak attitude ofpetiole of semi-erect semi-erect leaflet in relation to main axisInflorescence type mainly uniparous mainly uniparous type (thirdinflorescence) simple simple average number of flowers  8.2  7.6 ininflorescence (third inflorescence) leafy or “running” frequent frequentinflorescence (third inflorescence) Flower color yellow yellow calyxnormal (lobes awl normal (lobes awl shaped) shaped) calyx-lobes approx,equaling distinctly longer corolla than corolla corolla color yellowyellow style pubescence sparse sparse anthers all fused into tube allfused into tube fasciation (first flower absent absent of second orthird inflorescence) abscission layer absent (jointless) absent(jointless) Fruit surface smooth smooth base color (mature-green appleor medium apple or medium stage) green green pattern (mature-greenuniform green uniform green stage) green shoulder absent absentintensity of green color medium medium excluding shoulder (beforematurity) green stripes absent absent size large large ratiolength/diameter moderately moderately compressed compressed shape inlongitudinal oblate oblate section shape of blossom end flat indented toflat shape of stem end indented indented shape of pistil scar stellatestellate shape of transverse/cross round irregular section (third fruitof second or third cluster) ribbing at peduncle end weak weak depressionat peduncle end medium weak size of stem/peduncle scar medium small sizeof blossom scar small small point of detachment of fruit at calyx atcalyx at harvest (third fruit of attachment attachment second or thirdcluster) length (mature) (stem axis)  56.2  58.6 (mm) diameter at widestpoint  71.9  73.2 (mm) weight (mature) (g) 177.8 194.0 core presentpresent diameter of core in cross large medium section in relation tototal diameter number of locales three and four three and four color(full ripe) red red color at maturity red red flesh color (full-ripe)red/crimson red/crimson color of flesh (at maturity) red red glossinessof skin medium strong flesh color with lighter and with lighter anddarker areas in darker areas in walls walls locular gel color (table-red red ripe) firmness medium medium shelf life long medium time offlowering medium medium time of maturity medium medium ripening(blossom-to- uniform uniform stem axis) ripening (peripheral-to- insideout inside out central-radial axis) epidermis color yellow yellowepidermis easy-peel easy-peel epidermis texture average average pericarpthickness (mm)  8.6  9.1 thickness of pericarp very thick very thickPhenology seeding to 50% flowering  79.0  79.0 (1 open on 50% of plants)(days) seeding to once over 138.0 140.0 harvest (days) fruiting seasonlong long relative maturity in areas medium medium tested Adaptationculture greenhouse greenhouse principle use(s) fresh market fresh marketmachine harvest not adapted not adapted Chemistry and Composition pH 3.9  3.8 Titratable acidity, as %  6.2  6.6 citric acid Total solids(dry matter,  5.0  4.6 seeds and skin removed) (percentage totalcontent) Soluble solids as °Brix  6.0  5.4 These are typical values.Values may vary due to environment. Values that are substantiallyequivalent are within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of Tomato LineFIR-XM20-4542 CHARACTERISTIC FIR-XM20-4542 Marinice Seedling anthocyaninin hypocotyl of present present 2-15 cm seedling habit of 3-4 week oldnormal normal seedling Mature Plant height (cm) 217.6 181.3 height verylong long growth type indeterminate indeterminate form normal normalcanopy size (compared to medium medium others of similar type) habiterect erect Stem anthocyanin coloration of weak medium upper thirdlength of internode short short branching sparse sparse branching atcotyledon or first present present leafy node number of nodes betweenfirst 7 to 10 7 to 10 inflorescence number of nodes between  3.4  3.0early (first to second, second to third) inflorescences number of nodesbetween later  3.0  3.0 developing inflorescences pubescence on youngerstems sparsely hairy moderately hairy (scattered long hairs) Leaf type(mature leaf beneath the tomato tomato third inflorescence) type ofblade bipinnate bipinnate margins of major leaflets shallowly tootheddeeply toothed or (mature leaf beneath the third or scalloped cut, sps.toward inflorescence) base marginal rolling or wiltiness slight absent(mature leaf beneath the third inflorescence) onset of leaflet rolling(mature late season N/A leaf beneath the third inflorescence) surface ofmajor leaflets rugose rugose (mature leaf beneath the third (bumpy orveiny) (bumpy or veiny) inflorescence) pubescence (mature leaf normalnormal beneath the third inflorescence) attitude horizontal horizontallength long medium width broad broad size of leaflets large largeintensity of green color medium medium to dark glossiness medium mediumblistering weak weak attitude of petiole of leaflet in semi-erectsemi-erect relation to main axis Inflorescence type mainly uniparousmainly uniparous type (third inflorescence) simple simple average numberof flowers in  7.1  7.6 inflorescence (third inflorescence) leafy or“running” frequent frequent inflorescence (third inflorescence) Flowercolor yellow yellow calyx normal (lobes normal (lobes awl shaped) awlshaped) calyx-lobes approx, equaling distinctly longer corolla thancorolla corolla color yellow yellow style pubescence sparse sparseanthers all fused into tube all fused into tube fasciation (first flowerof absent absent second or third inflorescence) abscission layer absent(jointless) absent (jointless) Fruit surface smooth smooth base color(mature-green stage) apple or medium apple or medium green green pattern(mature-green stage) uniform green uniform green green shoulder absentabsent intensity of green color medium medium excluding shoulder (beforematurity) green stripes absent absent size medium large ratiolength/diameter medium moderately compressed shape in longitudinalsection oblong oblate shape of blossom end flat to pointed indented toflat shape of stem end indented indented shape of pistil scar stellatestellate shape of transverse/cross irregular irregular section (thirdfruit of second or third cluster) ribbing at peduncle end weak weakdepression at peduncle end weak weak size of stem/peduncle scar mediumsmall size of blossom scar small small point of detachment of fruit atat calyx at calyx harvest (third fruit of second or attachmentattachment third cluster) length (mature) (stem axis)  60.0  58.6 (mm)diameter at widest point (mm)  64.8  73.2 weight (mature) (g) 155.0194.0 core present present diameter of core in cross medium mediumsection in relation to total diameter number of locules two and threethree and four color (full ripe) red red color at maturity red red fleshcolor (full-ripe) red/crimson red/crimson color of flesh (at maturity)red red glossiness of skin weak strong flesh color with lighter and withlighter and darker areas in darker areas in walls walls locular gelcolor (table-ripe) red red firmness medium medium shelf life mediummedium time of flowering late medium time of maturity late mediumripening (blossom-to-stem blossom-to-stem uniform axis) end ripening(peripheral-to-central- inside out inside out radial axis) epidermiscolor yellow yellow epidermis easy-peel easy-peel epidermis textureaverage average pericarp thickness (mm)  9.2  9.1 thickness of pericarpvery thick very thick Phenology seeding to 50% flowering (1  83.0  79.0open on 50% of plants) (days) seeding to once over harvest 145.0 140.0(days) fruiting season long long relative maturity in areas testedmedium late medium Adaptation culture greenhouse greenhouse principleuse(s) fresh market fresh market machine harvest not adapted not adaptedChemistry and Composition pH  4.1  3.8 Titratable acidity, as % citric 5.6  6.6 acid Total solids (dry matter, seeds  5.2  4.6 and skinremoved) (percentage total content) Soluble solids as °Brix  6.4  5.4These are typical values. Values may vary due to environment. Valuesthat are substantially equivalent are within the scope of the invention.

TABLE 3 Physiological and Morphological Characteristics of Tomato LineFIR-XM20-4543 CHARACTERISTIC FIR-XM20-4543 Marinice Seedling anthocyaninin hypocotyl of present present 2-15 cm seedling habit of 3-4 week oldnormal normal seedling Mature Plant height (cm) 195.4 181.3 height longlong growth type indeterminate indeterminate form compact normal canopysize (compared to medium medium others of similar type) habit erecterect Stem anthocyanin coloration of weak medium upper third length ofinternode short short branching sparse sparse branching at cotyledon orfirst absent present leafy node number of nodes between first 7 to 10 7to 10 inflorescence number of nodes between  3.0  3.0 early (first tosecond, second to third) inflorescences number of nodes between later 3.0  3.0 developing inflorescences pubescence on younger stems sparselyhairy moderately (scattered long hairy hairs) Leaf type (mature leafbeneath the tomato tomato third inflorescence) type of blade bipinnatebipinnate margins of major leaflets shallowly toothed deeply toothed(mature leaf beneath the third or scalloped or cut, sps. inflorescence)toward base marginal rolling or wiltiness slight absent (mature leafbeneath the third inflorescence) onset of leaflet rolling (mature lateseason N/A leaf beneath the third inflorescence) surface of majorleaflets rugose rugose (mature leaf beneath the (bumpy or (bumpy orthird inflorescence) veiny) veiny) pubescence (mature leaf normal normalbeneath the third inflorescence) attitude semi-drooping horizontallength long medium width broad broad size of leaflets large largeintensity of green color medium medium to dark glossiness weak mediumblistering weak weak attitude of petiole of leaflet semi-erectsemi-erect in relation to main axis Inflorescence type mainly uniparousmainly uniparous type (third inflorescence) simple simple average numberof flowers  7.1  7.6 in inflorescence (third inflorescence) leafy or“running” frequent frequent inflorescence (third inflorescence) Flowercolor yellow yellow calyx normal (lobes normal (lobes awl shaped) awlshaped) calyx-lobes distinctly longer distinctly longer than corollathan corolla corolla color yellow yellow style pubescence sparse sparseanthers all fused into tube all fused into tube fasciation (first flowerof absent absent second or third inflorescence) abscission layer absent(jointless) absent (jointless) Fruit surface moderately rough smooth orribbed base color (mature-green apple or medium apple or medium stage)green green pattern (mature-green stage) uniform green uniform greengreen shoulder absent absent intensity of green color medium mediumexcluding shoulder (before maturity) green stripes absent absent sizevery large large ratio length/diameter very compressed moderately tomoderately compressed compressed shape in longitudinal section flattenedoblate shape of blossom end indented indented to flat shape of stem endindented indented shape of pistil scar irregular stellate shape oftransverse/cross irregular irregular section (third fruit of second orthird cluster) ribbing at peduncle end strong weak depression atpeduncle end strong weak size of stem/peduncle scar large small size ofblossom scar large small point of detachment of fruit at calyx at calyxat harvest (third fruit of attachment attachment second or thirdcluster) length (mature) (stem axis)  61.4  58.6 (mm) diameter at widestpoint (mm)  90.7  73.2 weight (mature) (g) 343.7 194.0 core presentpresent diameter of core in cross very large medium section in relationto total diameter number of locules more than six three and four color(full ripe) red red color at maturity red red flesh color (full-ripe)red/crimson red/crimson color of flesh (at maturity) red red glossinessof skin medium strong flesh color with lighter and with lighter anddarker areas in darker areas in walls walls locular gel color(table-ripe) red red firmness firm medium shelf life medium medium timeof flowering late medium time of maturity medium medium ripening(blossom-to-stem uniform uniform axis) ripening (peripheral-to-uniformity inside out central-radial axis) epidermis color yellow yellowepidermis easy-peel easy-peel epidermis texture average average pericarpthickness (mm)  10.2  9.1 thickness of pericarp very thick very thickPhenology seeding to 50% flowering (1  83.0  79.0 open on 50% of plants)(days) seeding to once over harvest 140.0 140.0 (days) fruiting seasonlong long relative maturity in areas medium medium tested Adaptationculture greenhouse greenhouse principle use(s) fresh market fresh marketmachine harvest not adapted not adapted Chemistry and Composition pH 3.8  3.8 Titratable acidity, as % citric  7.3  6.6 acid Total solids(dry matter, seeds  4.8  4.6 and skin removed) (percentage totalcontent) Soluble solids as °Brix  5.7  5.4 These are typical values.Values may vary due to environment. Values that are substantiallyequivalent are within the scope of the invention.

C. Breeding Tomato Plants

One aspect of the current invention concerns methods for producing seedof tomato hybrid DRTH1034 involving crossing tomato line FIR-XM20-4542and tomato line FIR-XM20-4543. Alternatively, in other embodiments ofthe invention, tomato hybrid DRTH1034, tomato line FIR-XM20-4542, ortomato line FIR-XM20-4543 may be crossed with itself or with any secondplant. Such methods can be used for propagation of tomato hybridDRTH1034, tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543 or canbe used to produce plants that are derived from tomato hybrid DRTH1034,tomato line FIR-XM20-4542, or tomato line FIR-XM20-4543. Plants derivedfrom tomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543 may be used, in certain embodiments, for the developmentof new tomato varieties.

The development of new varieties using one or more starting varieties iswell-known in the art. In accordance with the invention, novel varietiesmay be created by crossing tomato hybrid DRTH1034 followed by multiplegenerations of breeding according to such well-known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g., colchicine treatment). Alternatively, haploid embryos may begrown into haploid plants and treated to induce chromosome doubling. Ineither case, fertile homozygous plants are obtained. In accordance withthe invention, any of such techniques may be used in connection with aplant of the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withtomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543 for the purpose of developing novel tomato lines, it willtypically be preferred to choose those plants which either themselvesexhibit one or more selected desirable characteristics or which exhibitthe desired characteristic(s) when in hybrid combination. Examples ofdesirable traits may include, in specific embodiments, high seed yield,high seed germination, seedling vigor, high fruit yield, diseasetolerance or resistance, and adaptability for soil and climateconditions. Consumer-driven traits, such as a fruit shape, color,texture, and taste are other examples of traits that may be incorporatedinto new lines of tomato plants developed by this invention.

Delayed fruit ripening is a trait that is especially desirable in tomatoproduction, in that it provides a number of important benefits includingan increase in fruit shelf life, the ability to transport fruit longerdistances, the reduction of spoiling of fruit during transport orstorage, and an increase in the flexibility of harvest time. The abilityto delay harvest is especially useful for the processing industry astomato fruits may be picked in a single harvest. A number of genes havebeen identified as playing a role in fruit ripening in tomato. Mutationsin some of these genes were observed to be correlated with an extendedshelf life phenotype (Gang et al., Adv. Hort. Sci. 22: 54-62, 2008). Forexample, WO2010042865 discloses that certain mutations in the 2nd or 3rdexon in the non-ripening (NOR) gene can result in extended shelf lifephenotypes. These mutations are believed to result in an early stopcodon. It is therefore expected that any mutation resulting in an earlystop codon in an exon in the NOR gene, particularly a mutation resultingin an early stop codon in the 3rd exon, will result in a slower ripeningor an extended shelf life phenotype in tomato. A marker to select forthe unique mutation of each allele and to distinguish between thedifferent alleles of the NOR gene in a breeding program can be developedby methods known in the art.

D. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those tomato plants which are developed by a plantbreeding technique called backcrossing or by genetic engineering,wherein essentially all of the morphological and physiologicalcharacteristics of a variety are recovered or conserved in addition tothe single locus introduced into the variety via the backcrossing orgenetic engineering technique, respectively. By essentially all of themorphological and physiological characteristics, it is meant that thecharacteristics of a plant are recovered or conserved that are otherwisepresent when compared in the same environment, other than an occasionalvariant trait that might arise during backcrossing, introduction of atransgene, or application of a genetic engineering technique.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentaltomato plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental tomato plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a tomato plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny tomato plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of tomato the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiol., 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of tomato plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming, or otherwise disadvantageous. In addition,marker assisted selection may be used to identify plants comprisingdesirable genotypes at the seed, seedling, or plant stage, to identifyor assess the purity of a cultivar, to catalog the genetic diversity ofa germplasm collection, and to monitor specific alleles or haplotypeswithin an established cultivar.

Types of genetic markers which could be used in accordance with theinvention include, but are not necessarily limited to, Simple SequenceLength Polymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 18:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In particular embodiments of the invention, marker assisted selection isused to increase the efficiency of a backcrossing breeding scheme forproducing a tomato line comprising a desired trait. This technique iscommonly referred to as marker assisted backcrossing (MABC). Thistechnique is well-known in the art and may involve, for example, the useof three or more levels of selection, including foreground selection toidentity the presence of a desired locus, which may complement orreplace phenotype screening protocols; recombinant selection to minimizelinkage drag; and background selection to maximize recurrent parentgenome recovery.

E. Plants Derived by Genetic Engineering

Various genetic engineering technologies have been developed and may beused by those of skill in the art to introduce traits in plants. Incertain aspects of the claimed invention, traits are introduced intotomato plants via altering or introducing a single genetic locus ortransgene into the genome of a recited variety or progenitor thereof.Methods of genetic engineering to modify, delete, or insert genes andpolynucleotides into the genomic DNA of plants are well-known in theart.

In specific embodiments of the invention, improved tomato lines can becreated through the site-specific modification of a plant genome.Methods of genetic engineering include, for example, utilizingsequence-specific nucleases such as zinc-finger nucleases (see, forexample, U.S. Pat. Appl. Pub. No. 2011-0203012); engineered or nativemeganucleases; TALE-endonucleases (see, for example, U.S. Pat. Nos.8,586,363 and 9,181,535); and RNA-guided endonucleases, such as those ofthe CRISPR/Cas systems (see, for example, U.S. Pat. Nos. 8,697,359 and8,771,945 and U.S. Pat. Appl. Pub. No. 2014-0068797). One embodiment ofthe invention thus relates to utilizing a nuclease or any associatedprotein to carry out genome modification. This nuclease could beprovided heterologously within donor template DNA for templated-genomicediting or in a separate molecule or vector. A recombinant DNA constructmay also comprise a sequence encoding one or more guide RNAs to directthe nuclease to the site within the plant genome to be modified. Furthermethods for altering or introducing a single genetic locus include, forexample, utilizing single-stranded oligonucleotides to introduce basepair modifications in a tomato plant genome (see, for example Sauer etal., Plant Physiol, 170(4):1917-1928, 2016).

Methods for site-directed alteration or introduction of a single geneticlocus are well-known in the art and include those that utilizesequence-specific nucleases, such as the aforementioned, or complexes ofproteins and guide-RNA that cut genomic DNA to produce a double-strandbreak (DSB) or nick at a genetic locus. As is well-understood in theart, during the process of repairing the DSB or nick introduced by thenuclease enzyme, a donor template, transgene, or expression cassettepolynucleotide may become integrated into the genome at the site of theDSB or nick. The presence of homology arms in the DNA to be integratedmay promote the adoption and targeting of the insertion sequence intothe plant genome during the repair process through homologousrecombination or non-homologous end joining (NHEJ).

In another embodiment of the invention, genetic transformation may beused to insert a selected transgene into a plant of the invention ormay, alternatively, be used for the preparation of transgenes which canbe introduced by backcrossing. Methods for the transformation of plantsthat are well-known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation, anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Nat. Biotechnol., 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Nat. Biotechnol., 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, for example,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13:344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, for example, Odel et al., Nature, 313:810,1985), including in monocots (see, for example, Dekeyser et al., PlantCell, 2:591, 1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389,1990); a tandemly duplicated version of the CaMV 35S promoter, theenhanced 35S promoter (P-e35S); the nopaline synthase promoter (An etal., Plant Physiol., 88:547, 1988); the octopine synthase promoter(Fromm et al., Plant Cell, 1:977, 1989); the figwort mosaic virus(P-FMV) promoter as described in U.S. Pat. No. 5,378,619; an enhancedversion of the FMV promoter (P-eFMV) where the promoter sequence ofP-FMV is duplicated in tandem; the cauliflower mosaic virus 19Spromoter; a sugarcane bacilliform virus promoter; a commelina yellowmottle virus promoter; and other plant virus promoters known to expressin plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, or developmental signals can also beused for expression of an operably linked gene in plant cells, includingpromoters regulated by (1) heat (Callis et al., Plant Physiol., 88:965,1988), (2) light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., PlantCell, 1:471, 1989; maize rbcS promoter, Schaffner and Sheen, Plant Cell,3:997, 1991; or chlorophyll a/b-binding protein promoter, Simpson etal., EMBO J., 4:2723, 1985), (3) hormones, such as abscisic acid(Marcotte et al., Plant Cell, 1:969, 1989), (4) wounding (e.g., wunl,Siebertz et al., Plant Cell, 1:961, 1989); or (5) chemicals, such asmethyl jasmonate, salicylic acid, or Safener. It may also beadvantageous to employ organ-specific promoters (e.g., Roshal et al.,EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a tomato plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a tomato plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see, for example, Gibson and Shillito, Mol.Biotech., 7:125,1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

F. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a genetic locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F1), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions or transgenes from one geneticbackground into another.

Crossing: The mating of two parent plants.

Cross-Pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence, or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) Color Chart Value: The RHS Color Chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightness,and saturation. A color is precisely named by the RHS Color Chart byidentifying the group name, sheet number, and letter, e.g.,Yellow-Orange Group 19A or Red Group 41B.

Self-Pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing or genetic engineering ofa locus, wherein essentially all of the morphological and physiologicalcharacteristics of a tomato variety are recovered in addition to thecharacteristics of the single locus.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a tomato plant by transformation orsite-specific modification.

G. Deposit Information

A deposit of at least 625 seeds of tomato line FIR-XM20-4542 and tomatoline FIR-XM20-4543, disclosed above and recited in the claims, has beenmade with the Provasoli-Guillard National Center for Marine Algae andMicrobiota (NCMA), 60 Bigelow Drive, East Boothbay, Maine, 04544 USA.The date of deposit for those deposited seeds of tomato lineFIR-XM20-4542 and tomato line FIR-XM20-4543 is December 28, 2021. Theaccession numbers for those deposited seeds of tomato line FIR-XM20-4542and tomato line FIR-XM20-4543 are NCMA Accession No. 202112029 and NCMAAccession No. 202112030, respectively. Upon issuance of a patent, allrestrictions upon the deposits will be removed, and the deposits areintended to meet all of the requirements of 37 C.F.R. §§ 1.801-1.809.The deposits have been accepted under the Budapest Treaty and will bemaintained in the depository for a period of 30 years, 5 years after thelast request, or the effective life of the patent, whichever is longer,and will be replaced if necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed:
 1. A tomato plant comprising at least a first set ofthe chromosomes of tomato line FIR-XM20-4542 or tomato lineFIR-XM20-4543, a sample of seed of said lines having been depositedunder NCMA Accession No. 202112029 and NCMA Accession No. 202112030,respectively.
 2. A tomato seed that produces the plant of claim
 1. 3.The plant of claim 1, wherein the plant is a plant of said tomato lineFIR-XM20-4542 or tomato line FIR-XM20-4543.
 4. The plant of claim 1,wherein the plant is a plant of tomato hybrid DRTH1034.
 5. The seed ofclaim 2, wherein the seed is a seed of said tomato line FIR-XM20-4542 ortomato line FIR-XM20-4543.
 6. The seed of claim 2, wherein the seed is aseed of tomato hybrid DRTH1034.
 7. A plant part of the plant of claim 1,wherein the plant part comprises a cell of said plant.
 8. A tomato planthaving all of the physiological and morphological characteristics of theplant of claim
 1. 9. A tissue culture of regenerable cells of the plantof claim
 1. 10. A method of vegetatively propagating the plant of claim1, the method comprising the steps of: (a) collecting tissue capable ofbeing propagated from the plant of claim 1; and (b) propagating a tomatoplant from said tissue.
 11. A method of introducing a trait into atomato line, the method comprising: (a) utilizing as a recurrent parentthe plant of claim 1 by crossing said plant with a donor plant thatcomprises a trait to produce F₁ progeny; (b) selecting an F₁ progenythat comprises the trait; (c) backcrossing the selected F₁ progeny witha plant of the same line used as the recurrent parent in step (a) toproduce backcross progeny; (d) selecting a backcross progeny comprisingthe trait and otherwise comprising the morphological and physiologicalcharacteristics of the recurrent parent line used in step (a); and (e)repeating steps (c) and (d) three or more times to produce a selectedfourth or higher backcross progeny.
 12. A tomato plant produced by themethod of claim
 11. 13. A method of producing a tomato plant comprisingan added trait, the method comprising introducing a transgene conferringthe trait into the plant of claim
 1. 14. A tomato plant produced by themethod of claim
 13. 15. A tomato plant comprising at least a first setof the chromosomes of tomato line FIR-XM20-4542 or tomato lineFIR-XM20-4543, a sample of seed of said lines having been depositedunder NCMA Accession No. 202112029 and NCMA Accession No. 202112030,respectively, further comprising a transgene.
 16. The plant of claim 15,wherein the transgene confers a trait selected from the group consistingof male sterility, herbicide tolerance, insect resistance, pestresistance, disease resistance, modified fatty acid metabolism,environmental stress tolerance, modified carbohydrate metabolism, andmodified protein metabolism.
 17. A tomato plant comprising at least afirst set of the chromosomes of tomato line FIR-XM20-4542 or tomato lineFIR-XM20-4543, a sample of seed of said lines having been depositedunder NCMA Accession No. 202112029 and NCMA Accession No. 202112030,respectively, further comprising a single locus conversion.
 18. Theplant of claim 17, wherein the single locus conversion confers a traitselected from the group consisting of male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,modified fatty acid metabolism, environmental stress tolerance, modifiedcarbohydrate metabolism, and modified protein metabolism.
 19. A methodfor producing a seed of a tomato plant derived from at least one oftomato hybrid DRTH1034, tomato line FIR-XM20-4542, or tomato lineFIR-XM20-4543, the method comprising the steps of: (a) crossing theplant of claim 1 with itself or a different tomato plant; and (b)allowing a seed of a tomato hybrid DRTH1034-, tomato lineFIR-XM20-4542-, or tomato line FIR-XM20-4543-derived tomato plant toform.
 20. A method of producing a seed of a tomato hybrid DRTH1034-,tomato line FIR-XM20-4542-, or tomato line FIR-XM20-4543-derived tomatoplant, the method comprising the steps of: (a) producing a tomato hybridDRTH1034-, tomato line FIR-XM20-4542-, or tomato lineFIR-XM20-4543-derived tomato plant from a seed produced by crossing theplant of claim 1 with itself or a different tomato plant; and (b)crossing the tomato hybrid DRTH1034-, tomato line FIR-XM20-4542-, ortomato line FIR-XM20-4543-derived tomato plant with itself or adifferent tomato plant to obtain a seed of a further tomato hybridDRTH1034-, tomato line FIR-XM20-4542-, or tomato lineFIR-XM20-4543-derived tomato plant.
 21. The method of claim 20, themethod further comprising repeating said producing and crossing steps of(a) and (b) using the seed from said step (b) for producing the tomatoplant according to step (a) for at least one generation to produce aseed of an additional tomato hybrid DRTH1034-, tomato lineFIR-XM20-4542-, or tomato line FIR-XM20-4543-derived tomato plant.
 22. Amethod of producing a tomato fruit, the method comprising: (a) obtainingthe plant of claim 1, wherein the plant has been cultivated to maturity;and (b) collecting a tomato fruit from the plant.